MassIVE MSV000097984

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GNPS - Untargeted Metabolomic Profiling of Streptomyces and Nocardia from Oligotrophic Ecosystems

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Description

We conducted an untargeted metabolomic analysis of Streptomyces and Nocardia strains isolated from two contrasting oligotrophic environments: Cuatro Cienegas and Calakmul. Metabolomic profiling was performed using liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-HR-MS/MS). Data acquisition was carried out in positive ionization mode under a Data-Dependent Acquisition (DDA) strategy. The analysis included samples cultivated under two distinct culture conditions: ISP2 and ISP5 media. Metabolites were extracted using a solvent mixture of acetonitrile, methanol, and ethyl acetate in a 1:1:1 ratio [doi:10.25345/C5PG1J16W] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Actinobacteria ; Microbial Ecology ; Chemical Ecology ; DatasetType:Metabolomics

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Principal Investigators:
(in alphabetical order) 		Aldo Moreno Ulloa, Centro de Investigacion Cientifica y de Educacion Superior de Ensenada, Mexico
Submitting User: froz9
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Identification Results
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GNPS content goes here (MSV000097984 [task=8ff83c737e0b4786b377cbfc753a348b])
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.