MassIVE MSV000093990

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EDC-3 and EDC-4 Regulate Embryonic mRNA Clearance and Biomolecular Condensate Specialization

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Description

Animal development is dictated by the selective and timely decay of mRNAs in developmental transitions. The implication of mRNA decapping scaffold proteins in these processes was unknown. This study delineates the roles and interactions of the DCAP-2 decapping scaffolds EDC-3 and EDC-4 in the embryonic development of C. elegans. EDC-3 facilitates the timely removal of specific embryonic mRNAs, including ifet-1, car-1, and cgh-1, reducing their expression, and preventing excessive accumulation of DCAP-2 condensates in somatic cells. We further uncover a novel role for EDC-3 in defining the biochemical boundaries between P-bodies, P-granules, and stress granules. Lastly, we show that EDC-4 counteracts EDC-3 and mediates the assembly of DCAP-2 with the GID (CTLH) complex, a ubiquitin ligase involved in maternal-to-zygotic transition (MZT). Our findings refine a model wherein multiple RNA decay mechanisms temporally partake in the clearance of maternal and zygotic mRNAs throughout embryonic development. [doi:10.25345/C5K06XB8K] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Decapping, P bodies, DCAP-2, EDC-3, EDC-4

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Principal Investigators:
(in alphabetical order) 		Thomas F Duchaine, Department of Biochemistry, McGill University, Canada
Submitting User: adarshmayank
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