MassIVE MSV000095941

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Vehicle versus FASN-inhibited human metastatic breast cancer global whole cell lysate and lipid droplets

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Description

Metastatic breast cancer cells (MCF10CA1a) were treated with vehicle (DMSO) or 20 uM of Fatty Acid Synthase (FASN) inhibitor TVB-3166 for three days. Untargeted global proteomics (Tryp/Lys-C; Lumos) was performed on the whole cell lysates, as well as isolated lipid droplet fractions from each lysate. All samples were prepared in the same manner, including lipid extraction prior to protein digestion. [doi:10.25345/C5H41JZ8Z] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: breast cancer ; lipid metabolism ; fatty acid synthase ; FASN ; TVB-3166 ; cancer metabolism ; MCF10CA1a ; lipid droplet ; fatty acid synthesis

Contact

Principal Investigators:
(in alphabetical order) 		Chaylen J Andolino, Purdue University, United States
Dorothy Teegarden, Purdue University, USA
Submitting User: candolin
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