MassIVE MSV000085388

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Middle-up analysis of polyclonal IgG-Fc

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Description

Middle-up strategy for the analysis of the fragment crystallizable (Fc) region of human plasma immunoglobulins G (IgGs). The use of hydrophilic interaction liquid chromatography (HILIC) and capillary electrophoresis (CE) coupled to mass spectrometry (MS) allowed to get a comprenhesive view of the N-glycosylation and other PTMs for each subclass and allotypes. [doi:10.25345/C5K11H] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Immunoglobulin G ; allotype ; fragment crystallizable ; N-glycosylation ; hydrophilic liquid chromatography ; capillary electrophoresis

Contact

Principal Investigators:
(in alphabetical order) 		Manfred Wuhrer, Leiden University Medical Center, Netherlands
Submitting User: tpsenard

Publications

Sénard T, Gargano AFG, Falck D, de Taeye SW, Rispens T, Vidarsson G, Wuhrer M, Somsen GW, Domínguez-Vega E.
MS-Based Allotype-Specific Analysis of Polyclonal IgG-Fc N-Glycosylation.
Front Immunol. Epub 2020 Aug 21.

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