MassIVE MSV000094967

Partial Public PXD052966

GSK3 inhibition reduces ECM production and prevents age-related macular degeneration-like pathology

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Description

All samples originated from ARPE-19 cultured cells. Cells were treated with either vehicle (DMSO, 1:1000) or CHIR99021 (1 uM) in serum free media for 7 days followed by harvesting the conditioned lysate, concentration using an Amicon filter, and loading onto an SDS-PAGE gel. Total protein (unsolved, in the stacking gel) was stained with Coomassie Blue and the gel bands containing total secreted protein were excised and submitted to the Proteomics Core (UT Southwestern). [doi:10.25345/C5G737F80] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: EFEMP1 ; fibulin-3 ; extracellular matrix ; CHIR99021 ; Doyne Honeycomb Retinal Dystrophy ; Malattia Leventinese ; age-related macular degeneration ; GSK3 ; ARPE-19

Contact

Principal Investigators:
(in alphabetical order) 		John D. Hulleman, Ph.D., University of Minnesota, USA
Submitting User: alemoff
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