MassIVE MSV000089642

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Mablango_ClpP_HYTANE_P124_Lumos

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Description

This dataset consists of 6 raw MS files, acquired on Thermo Fusion Lumos mass spectrometer operated in Data Dependent Acquisition mode. Samples were generated by Keith Wong. Sample processing and mass spectrometry acquisition was performed by Cassandra Wong. Data analysis was performed by Cassandra Wong, Shen Zhang and Keith Wong. The files are associated with a manuscript submitted for publication by Mark Mabanglo et al. The main goal of this paper was to demonstrate the use of novel imipridone derived compounds to induce selective cancer cell lethality via dysregulation of ClpP in cancer cells. Walid Houry is the corresponding author of the manuscript (walid.houry@utoronto.ca); Anne-Claude Gingras should be contacted for questions on this dataset (gingras@lunenfeld.ca) This submission is associated with 4 Supplementary Files (in addition to this README file) Metadata describes the composition of this dataset. Peptides lists all peptide identification evidence. Proteins lists all protein identification evidence. Pvalue_peptides lists all p-values calculated between conditions for peptides. [doi:10.25345/C5ZG6GB6G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: ClpP ; Imipridones ; cancer ; HYTANE

Contact

Principal Investigators:
(in alphabetical order) 		Anne-Claude Gingras, LTRI, Canada
Submitting User: gingraslab

Publications

Mark F Mabanglo 1, Keith S Wong 1, Marim M Barghash 1, Elisa Leung 1, Stephanie H W Chuang 1, Afshan Ardalan 1, Emily M Majaesic 2, Cassandra J Wong 3, Shen Zhang 4, Henk Lang 5, Donald S Karanewsky 5, Andrew A Iwanowicz 5, Lee M Graves 6, Edwin J Iwanowicz 5, Anne-Claude Gingras 7, Walid A Houry 8.
Potent ClpP agonists with anticancer properties bind with improved structural complementarity and alter the mitochondrial N-terminome.
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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.