MassIVE MSV000080719

Imported Reanalysis Dataset Public PXD002775

Extrarenal ?-klotho expression in human tissues

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Description

?-Klotho has emerged as a powerful regulator of the ageing process. To-date, the expression profile of ?-Klotho in human tissues is unknown and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system-wide tissue expression of transmembrane ?-Klotho in humans. We have employed next generation targeted proteomic analysis using Parallel Reaction Monitoring (PRM) in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human ?-Klotho expression in health. Results: The distribution of ?-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive and neuronal tissues was first identified by immunohistochemistry. Kidney tissues showed strong ?-Klotho expression, while liver did not reveal a detectable signal. These results were next confirmed by western blotting of both whole tissues and primary cells. To validate our antibody-based results, ?-Klotho expressing tissues were subjected to PRM mass spectrometry identifying peptides specific for the full length, transmembrane ?-Klotho isoform. Conclusions: The data presented confirms ?-Klotho expression in the kidney tubule and in artery, and provides evidence of ?-Klotho expression across organ systems and cell-types that have not previously been described in humans. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Human ; Klotho ; Ontogeny ; PRM

Contact

Principal Investigators:
(in alphabetical order) 		Kathryn Lilley, Cambridge Centre for Proteomics University of Cambridge, N/A
Submitting User: ccms

Publications

Lim K, Groen A, Molostvov G, Lu T, Lilley KS, Snead D, James S, Wilkinson IB, Ting S, Hsiao LL, Hiemstra TF, Zehnder D.
?-Klotho Expression in Human Tissues.
J. Clin. Endocrinol. Metab. 2015 Oct;100(10):E1308-18. Epub 2015 Aug 17.

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