MassIVE MSV000091856

Complete Public PXD041980

IEX fractionated proteome of pig tracheal cilia extract

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Description

Cilia from two fresh pig tracheas (Sus scrofa) were removed by calcium shock and proteins extracted in buffer containing 1% NP40. Extract was diluted to reduce salts and fractionated by HPLC on a mixed bed ion exchange column. Fractions were reduced/alkylated and digested with trypsin for analysis by LC-MS/MS on an orbitrap fusion Lumos using a 75 minute top speed DDA method and HCD. [doi:10.25345/C5M03Z72X] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: pig, sus scrofa, trachea, cilia, LECA, IEX, orbitrap fusion Lumos

Contact

Principal Investigators:
(in alphabetical order) 		Edward Marcotte, University of Texas-Austin, United States
Submitting User: OpheliaP
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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