Cilia from two fresh pig tracheas (Sus scrofa) were removed by calcium shock and proteins extracted in buffer containing 1% NP40. Extract was diluted to reduce salts and fractionated by HPLC on a mixed bed ion exchange column. Fractions were reduced/alkylated and digested with trypsin for analysis by LC-MS/MS on an orbitrap fusion Lumos using a 75 minute top speed DDA method and HCD.
[doi:10.25345/C5M03Z72X]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: pig, sus scrofa, trachea, cilia, LECA, IEX, orbitrap fusion Lumos
Principal Investigators: (in alphabetical order) |
Edward Marcotte, University of Texas-Austin, United States |
Submitting User: | OpheliaP |
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