MassIVE MSV000080807

Partial Public PXD006220

MudPIT analyses of the human proteins biotinylated by proximity to biotin ligase (BioID) fused to the N-terminus of activators of the dynein/dynactin motor

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Description

To capture the spatial organization of the dynein/dynactin motor, we attached a promiscuous biotin ligase (BioID) to 6 distinct activators of the dynein machinery motile activity. BioID experiments were performed with stable HEK293 cell lines expressing full length BICD1, BICD2, HOOK1, HOOK3, NIN and NINL with BioID tags at their N-termini. For BioID experiments, cells were lysed in the presence of detergents to disrupt the dynein/ dynactin complex, allowing the identification of proteins that were proximal to the tagged activator prior to cell lysis. After purification of biotinylated proteins on streptavidin-conjugated beads, the eluates were precipitated with TCA. After washing with acetone, the protein mixtures were digested with endoproteinase Lys-C and trypsin (Promega) and analyzed by MudPIT. We performed BioID purification followed by MudPIT in quadruplicate and used a label-free quantitative proteomics approach to calculate the enrichment of each identified protein relative to BioID control replicates. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Cytoplasmic dynein-1 ; dynactin ; activator ; coiled-coil ; cargo specificity

Contact

Principal Investigators:
(in alphabetical order) 		Laurence Florens, The Stowers Institute for Medical Research, USA
Submitting User: laflorens

Publications

Redwine WB, DeSantis ME, Hollyer I, Htet ZM, Tran PT, Swanson SK, Florens L, Washburn MP, Reck-Peterson SL.
The human cytoplasmic dynein interactome reveals novel activators of motility.
Elife. Epub 2017 Jul 18.

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