MassIVE MSV000094034

Complete Public PXD049256

2H-Thiopyran-2-Thione Sulfine, a Compound For Converting H2S to HSOH/H2S2 and Increasing Intracellular Sulfane Sulfur Levels

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Description

Reactive sulfane sulfur species such as persulfides (RSSH) and H2S2 are important redox regulators and closely linked to H2S signaling. However, the study of these species is still challenging due to their instability, high reactivity, and the lack of suitable donors to produce these species. Herein we report a unique compound, 2H-thiopyran-2-thione sulfine (TTS), which can specifically convert H2S to HSOH, and then to H2S2 in the presence of excess H2S. Meanwhile, the reaction product 2H-thiopyran-2-thione (TT) can be oxidized to reform TTS by biological oxidants. TTS can be conjugated to proteins to achieve specific delivery, and the combination of TTS and H2S leads to highly efficient protein persulfidation. When TTS is applied in conjunction with established H2S donors, the corresponding donors of H2S2 (or its equivalents) are obtained. Cell-based studies reveal that TTS can effectively increase intracellular sulfane sulfur levels and compensate for certain aspects of sulfide:quinone oxidoreductase (SQR) deficiency. Sample 1: GAPDH-SH + TTS + HPE-IAM; Sample 2: GAPDH-SH + TTS + Na2S + HPE-IAM; Sample 3: GAPDH-SH + TTS + Na2S + HPE-IAM [doi:10.25345/C50V89T71] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: GAPDH ; persulfidation

Contact

Principal Investigators:
(in alphabetical order) 		Wei-Jun Qian, Pacific Northwest National Laboratory, United States
Submitting User: alchemistmatt

Publications

Cui Q, Shieh M, Pan TW, Nishimura A, Matsunaga T, Kelly SS, Xu S, Jung M, Ogata S, Morita M, Yoshitake J, Chen X, Robinson JR, Qian WJ, Nishida M, Akaike T, Xian M.
2H-Thiopyran-2-thione sulfine, a compound for converting H2S to HSOH/H2S2 and increasing intracellular sulfane sulfur levels.
Nat Commun. 2024 Mar 19;15(1):2453. Epub 2024 Mar 19.

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