MassIVE MSV000083023

Complete Public PXD011340

The mRNA-bound proteome of Leishmania mexicana is stage-regulated with distinct transcript target pools

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Description

Leishmania species parasite infections, termed the leishmaniases, cause significant global infectious disease burden. The lifecycle of the parasite embodies three main stages that require precise coordination of gene regulation to survive drastic environmental shifts transitioning from sandfly to mammalian hosts. Constitutive transcription in these kinetoplastid parasites is overwhelmingly reliant on post-transcriptional mechanisms, yet strikingly few Leishmania trans-regulator proteins are known. Utilizing optimised crosslinking and in-depth, quantified mass spectrometry, we present a comprehensive analysis of over 1,400 mRNA binding proteins (mRBPs) and whole cell proteomes from the three main lifecycle stages. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: mRNA-bound proteome ; mRNA binding proteins ; mRBPs ; mRBPome ; parasite lifecycle

Contact

Principal Investigators:
(in alphabetical order) 		Dr. Pegine Walrad, University of York, United Kingdom
Submitting User: aad100

Publications

De Pablos LM, Ferreira TR, Dowle A, Forrester S, Parry E, Newling K, Walrad PB.
The mRNA-bound proteome of Leishmania mexicana: novel genetic insight into an ancient parasite.
Mol. Cell Proteomics. Epub 2019 Apr 4.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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