MassIVE MSV000100409

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Degradation of native and labelled linolenic acid by N. europaea

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Description

Nitrosomonas europaea Nm50 BNI degradation experiment:
- concentrate ~ 600 ml culture
- resuspend to make motherculture - 270 ml (need 255 for experiments)
- let acclimate back to 26C for ~20min- 1h without substrate
- add 1 mM NH4Cl (final concentration) to mother culture bottle:
- take T0 mother culture; take T1 mother culture (cca 20 min); T2 mother culture (40min);
split to treatments after 60 min

Treatments:
prepare 150 ul Linolenic acid (LNL) mix (50% unlabeled; 50% 13C LNL) on ice; after using
120 for inoculation of treatments; freeze the 30 ul mix @ -80
prepare just medium with 0.5 mM NH4

Biotic:
LNL; 8 ul of (50% unlabeled; 50% 13C LNL mix) - 5 replicates; 17 ml culture each (5)
PHA-LNL - 1 mM PHA+8 ul of (50% unlabeled; 50% 13C LNL mix) - 5 replicates; 17 ml
culture each (5)
control - nothing added - 5 replicates; 17 ml culture each (5)

Abiotic:
LNL - 8 ul of (50% unlabeled; 50% 13C LNL mix) - 5 replicates; 17 ml medium with 0.5 mM
NH4+ (5)

Sampling every 30-45 min; 5 timepoints

A: LE + culture
B: PHA-LE + culture
C: culture only
D: LE in media only

LC-HRMS according to 10.1007/s00216-025-05818-y
[doi:10.25345/C5P26QH3J] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: 13C ; stable isotope ; DatasetType:Metabolomics

Contact

Principal Investigators:
(in alphabetical order) 		christoph bueschl, BOKU university, Austria
Submitting User: christophb
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