MassIVE MSV000090330

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The mismatch recognition protein MutSalpha promotes nascent strand degradation at stalled replication forks

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Description

Regular iPOND: 707789: C3-4 EdU, 707790: C3-4 Thymidine chase, 707791: A3-6 EdU, 707792: A3-6 Thymidine chase. SILAC iPOND: 1002641. Regular iPOND: WT MEF cell line (C3-4) and P286R MEF cells (A3-6) were labeled with EdU with (C3-4/A3-6 Thymidine chase) or without thymidine chase (C3-4/A3-6 EdU). Click reaction was performed to link a biotin to the EdU followed by pull down with streptavidin beads. SILAC iPOND: P286R MEF cells (abundance 1002641 Light) were cultured in "light" medium containing [12C14N]-L-lysine and [12C14N]-L-arginine, and WT MEF cells (Abundance 1002641 Heavy) were cultured in "heavy" medium containing [13C15N]-L-lysine [13C15N]-L-arginine. After more than 99% cells were labeled with isotopes, equal amount of WT and P286R cells were combined followed by EdU labeling and click reaction. Proteins captured were dissolved on SDS/PAGE and subjected to MS analysis. [doi:10.25345/C5RJ49022] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: MutSalpha ; MRE11 ; replication fork stability ; nascent strand degradation ; chromosome instability

Contact

Principal Investigators:
(in alphabetical order) 		Guo-Min Li, University of Texas Southwestern Medical Center, United States
Submitting User: jjotto
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