MassIVE MSV000087935

Partial Public

GNPS DOM Interlab Ring Trial LCMS - Lab 26

Description

DOM Interlab LCMS Samples from Lab 26. Infused with a Thermo Dionex 3000 RSLC using the loading pump at 400 ul/min with gradient as prescribed onto a Hypersil Gold C18 column (2.1x100mm, 1.9um particle size). Analysed with a Thermo Orbitrap Exploris 480 using HESI source in positive and negative mode with MS1 and MS2 methods. Note: sample was used up prematurely so duplicate injection replicates only for Pos MS1 and MS2, and neg MS2. Negative MS1 had triplicate injections. Internal calibration (IC) source enabled for improved mass accuracy. Sample infusion order: Neg MS1 rep 1, Neg MS2 rep 2, Pos MS1 rep 1, Pos MS2 rep1, (and then repeated). Within each polarity/MS group, samples were randomised - and differently between each group. No-injection blanks were run between group, and QCs and solvent and PPL SPE blanks were analyzed before the first rep of each polarity/MS setting. [doi:10.25345/C56R80] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: LCMS ; DOM ; Algal ; Interlab

Contact

Principal Investigators:
(in alphabetical order)
Will Kew, PNNL, United States
Submitting User: williamkew
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Spectra:
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Experimental Design
    Conditions:
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Identification Results
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    Variant Peptides:
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Quantification Results
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    Quantified Proteins:
 
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GNPS content goes here (MSV000087935 [task=a1ed0b75fd1740ec8b3ebc39779eca73])
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.