MassIVE MSV000095128

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Enhanced payload localization in antibody-drug conjugates using a middle-down mass spectrometry approach with proton transfer charge reduction

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Description

Here, we present an MD MS strategy relying on the use of PTCR to investigate an ADC with variable drug-to-antibody ratio, targeting the unambiguous localization of payload conjugation sites. Unlike traditional tandem MS experiments (MS2), which were largely ineffective, a single PTCR-based experiment (MS3) proved to be sufficient to achieve this goal across all modified ADC subunits. [doi:10.25345/C5NZ8120D] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: middle down mass spectrometry ; antibody drug conjugate ; biotherapeutics ; proton transfer charge reduction ; electron transfer dissociation ; ultraviolet photodissociation ; Orbitrap

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Principal Investigators:
(in alphabetical order) 		Luca Fornelli, University of Oklahoma, United States
Submitting User: Fornelli_Lab
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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