MassIVE MSV000082920

Partial Public PXD011028

Cytosolic proteome of Staphylococcus aureus HG001 post internalization in bronchial epithelial cells - S. aureus dataset

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Description

The data set consists of 32 S. aureus cytosolic samples that were collected by GFP supported cell sorting during infection in lung epithelial cells. The data include 8 time points (2.5 h, 6.5 h , 24 h, 48 h, 72 h, and 96 h post infection plus exponential and non-adherent control) that were collected out of four biological replicates. DIA data are provided. The respective spectral library was already published (Michalik et al., 2017, Sci Rep. doi: 10.1038/s41598-017-10059-w. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: S. aureus, human bronchial epithelial cells, host-pathogen interaction, internalization, proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Prof. Dr. Uwe Voelker, University Medicine Greifswald, Germany
Submitting User: surmannk

Publications

Palma Medina LM, Becker AK, Michalik S, Yedavally H, Raineri EJM, Hildebrandt P, Gesell Salazar M, Surmann K, Pförtner H, Mekonnen SA, Salvati A, Kaderali L, van Dijl JM, Völker U.
Metabolic cross-talk between human bronchial epithelial cells and internalized Staphylococcus aureus as a driver for infection.
Mol. Cell Proteomics. Epub 2019 Feb 26.

palma medina, surmann.
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.