MassIVE MSV000085567

Partial Public PXD019685

Proteomic portraits of human and porcine spinal cord injury define cross-species biomarkers of injury severity and recovery

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Description

Despite the emergence of promising therapeutic approaches in preclinical studies, the failure of large-scale clinical trials leaves clinicians without effective treatments for acute spinal cord injury (SCI). These trials are hindered by their reliance on detailed neurological examinations to establish outcomes, which inflate the time and resources required for completion. Moreover, therapeutic development takes place in animal models whose relevance to human injury remains unclear. Here, we address these challenges through targeted proteomic analyses of CSF and serum samples from 111 acute SCI patients and, in parallel, a large animal (porcine) model of SCI. We develop and validate protein biomarkers of injury severity and recovery, including a prognostic model of neurological improvement at six months with an AUC of 0.91. Through comparative proteomic analyses, we dissect conserved and divergent aspects of the SCI response, and establish the CSF abundance of glial fibrillary acidic protein (GFAP) as a biochemical outcome measure in both human and pig. Our work defines new resources to catalyze translation by facilitating the evaluation of novel SCI therapies, while also providing a resource from which to direct future preclinical efforts. [doi:10.25345/C5H13N] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: CSF ; cross-species ; spinal cord injury

Contact

Principal Investigators:
(in alphabetical order) 		Dr. Brian K. Kwon, International Collaboration on Repair Discoveries (ICORD), University of British Columbia, Vancouver, BC, Canada
Submitting User: Rogy
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