This data set is downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS126 Abstract:Resveratrol (RESV) is a plant polyphenol, which is thought to have beneficial metabolic effects in laboratory animals as well as in humans. Following oral administration, RESV is immediately catabolized, resulting in low bioavailability. This study compared RESV metabolites and their tissue distribution after oral uptake and skin absorption. Metabolomic analysis of various mouse tissues revealed that RESV can be absorbed and metabolized through skin. We detected sulfated and glucuronidated RESV metabolites, as well as dihydroresveratrol. These metabolites are thought to have lower pharmacological activity than RESV. Similar quantities of most RESV metabolites were observed 4 h after oral or skin administration, except that glucuronidated RESV metabolites were more abundant in skin after topical RESV application than after oral administration. This result is consistent with our finding of glucuronidated RESV metabolites in cultured skin cells. RESV applied to mouse ears significantly suppressed inflammation in the TPA inflammation model. The skin absorption route could be a complementary, potent way to achieve therapeutic effects with RESV.
One mg of RESV dissolved in ethanol was applied directly (Et) or mixed with hydrophilic ointment (HO), macrogol (Ma) or CMC gel (CMC), and swabbed on mouse dorsal skin. After 4 h mice were sacrificed, metabolites were extracted from tissues and analyzed by LC-MS. Peak areas of metabolites were normalized using peak areas of spiked internal standards (10 nmol 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and piperazine-N,N’-bis(2-ethanesulfonic acid) (PIPES). [dataset license: CC0 1.0 Universal (CC0 1.0)]
Principal Investigators: (in alphabetical order) |
Murakami, Graduate School of Medicine, Kyoto University, jp |
Submitting User: | rsilva |
Number of Files: | |
Total Size: | |
Spectra: | |
Subscribers: | |
Owner | Reanalyses | |
---|---|---|
Experimental Design | ||
Conditions:
![]() |
||
Biological Replicates:
![]() |
||
Technical Replicates:
![]() |
||
Identification Results | ||
Proteins (Human, Remapped):
![]() |
||
Proteins (Reported):
![]() |
||
Peptides:
![]() |
||
Variant Peptides:
![]() |
||
PSMs:
![]() |
||
Quantification Results | ||
Differential Proteins:
![]() |
||
Quantified Proteins:
![]() |
||
Browse Dataset Files | |
Browse Metadata | |
FTP Download Link (click to copy):
|