MassIVE MSV000097328

Partial Public PXD061853

Integrated genomics and proteomics analysis uncovers the oxidative stress role of miR-10b targets in healthy and diabetic human limbal epithelial cells

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Description

The corneal epithelium is maintained by limbal epithelial stem cells (LESCs) and is largely responsible for corneal optical transparency and protection by continuously renewing population of corneal epithelial cells. Diabetes mellitus (DM) affects all structures of the eye including the cornea, which can result in delayed wound healing and potential vision loss. MicroRNAs (miRNAs) are short non-coding oligonucleotides that regulate various cellular functions, including oxidative stress response, by repressing protein translation. MiR-10b-5p was previously identified to be upregulated in diabetic vs. non-diabetic limbal cells, and our purpose was to understand the role of miR-10b-5p in human limbal epithelial cells in healthy and diabetic conditions. Through integrated transcriptomic and proteomic analyses, we identified GCLM and LANCL1 as key miR-10b-5p targets, revealing its profound impact on glutathione metabolism, sulfur compound biosynthesis processes, and antioxidant defenses. Our findings suggest that overexpression of miR-10b disrupts redox balance, which potentially leads to heightened oxidative stress and increased cellular vulnerability in diabetic corneas. Understanding miR-10b function in corneal epithelial cells may pave the way for novel therapeutic strategies to mitigate oxidative stress and normalize corneal health in diabetic patients. [doi:10.25345/C5222RJ6R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: diabetic cornea ; limbal stem cells ; miR-10b ; oxidative stress ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Mehrnoosh Saghizadeh Ghiam, Cedars-Sinai Medical Center, USA
Submitting User: NivedaS5
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