MassIVE MSV000089252

Partial Public PXD033208

Human HEK293T and Pig cardiac top-down proteomes from Bruker maXis II

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Description

These data, derived from PXD019368 (PMID 33231430) and PXD010825 (PMID 30988469) represent a test of deconvolution algorithms for top-down proteomics experiments on the Bruker maXis II Q-TOF. Three LC-MS/MS experiments are available from pig cardiac tissue in both raw .d directories and in deconvolved msAlign files produced by Bruker DataAnalysis 5.3, TopFD 1.4.13, FLASHDeconv 2.0, and Mascot Distiller 2.8. Eight LC-MS/MS experiments are available from human HEK293T cell lysates, with four produced using Triton and four produced using Tergitol. The inferred precursor charges, precursor masses, and numbers of tandem mass spectra diverge considerably depending upon deconvolution algorithm. [doi:10.25345/C5R49GD28] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: top-down ; Q-TOF ; deconvolution

Contact

Principal Investigators:
(in alphabetical order) 		Ying Ge, University of Wisconsin-Madison, United States of America
Submitting User: dtabb73

Publications

Brown KA, Chen B, Guardado-Alvarez TM, Lin Z, Hwang L, Ayaz-Guner S, Jin S, Ge Y.
A photocleavable surfactant for top-down proteomics.
Nat Methods. 2019 May;16(5):417-420. Epub 2019 Apr 15.

Brown KA, Tucholski T, Alpert AJ, Eken C, Wesemann L, Kyrvasilis A, Jin S, Ge Y.
Top-Down Proteomics of Endogenous Membrane Proteins Enabled by Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry.
Anal Chem. 2020 Dec 15;92(24):15726-15735. Epub 2020 Nov 24.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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