MassIVE MSV000085616

Partial Public

Changes in the ECM during mouse kidney development

Description

Wildtype C57BL/6 mice kidneys from E14.5, E18.5, P3, and adult were isolated. Proteins were sequentially fractionated and the CS and IN fractions were processed and analyzed using mass spectrometry. [doi:10.25345/C5GQ6C] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: interstitial matrix, basement membrane, mass spectrometry, 3D imaging, elastin-microfibril axis

Contact

Principal Investigators:
(in alphabetical order)
Sarah Calve, University of Colorado Boulder, United States
Submitting User: snlipp

Publications

Lipp SN, Jacobson KR, Hains DS, Schwarderer AL, Calve S.
3D Mapping Reveals a Complex and Transient Interstitial Matrix During Murine Kidney Development.
J Am Soc Nephrol. Epub 2021 Apr 19.

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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.