MassIVE MSV000082189

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GNPS - Butyrate Suppresses Proliferation of Colon Cancer Cells via Targeting Pyruvate Kinase M2 and Metabolic Reprogramming

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Description

How butyrate interrupts the metabolism of cancer cells and leads to the suppression of cell proliferation remains unclear. We employed a metabolomics-proteomics combined approach to explore the link between butyrate-mediated proliferation arrest and cell metabolism. Metabolomics study revealed a remodeled metabolic profile with pronounced accumulation of pyruvate. A proteomics-based approach reveals PKM2 as a direct target of butyrate. it activates PKM2 via promoting its dephosphorylation and tetramerization.Our study provides a mechanistic link between PKM2-induced metabolic remodeling and the anti-tumorigenic function of butyrate. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Metabolomics, Proteomics, Pyruvate kinase M2, Butyrate, Colorectal cancer, Metabolic reprogramming.

Contact

Principal Investigators:
(in alphabetical order) 		Hui Ye, China Pharmaceutical University, China
Submitting User: Butyrate_CPU2018
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GNPS content goes here (MSV000082189 [task=ac70452569f54e2589b70feb706f15bf])
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.