How butyrate interrupts the metabolism of cancer cells and leads to the suppression of cell proliferation remains unclear. We employed a metabolomics-proteomics combined approach to explore the link between butyrate-mediated proliferation arrest and cell metabolism. Metabolomics study revealed a remodeled metabolic profile with pronounced accumulation of pyruvate. A proteomics-based approach reveals PKM2 as a direct target of butyrate. it activates PKM2 via promoting its dephosphorylation and tetramerization.Our study provides a mechanistic link between PKM2-induced metabolic remodeling and the anti-tumorigenic function of butyrate.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Metabolomics, Proteomics, Pyruvate kinase M2, Butyrate, Colorectal cancer, Metabolic reprogramming.
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Hui Ye, China Pharmaceutical University, China |
Submitting User: | Butyrate_CPU2018 |
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