MassIVE MSV000093427

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A fast and sensitive size exclusion chromatography method for plasma extracellular vesicle proteomic analysis

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Description

Proteomics data from SEC-fractionated human plasma; commercial human plasma from females. Clinical samples provided from 9 individuals before or after 45 minutes of intense exercise. Samples were SEC fractionated, TCEP reduced, and IAA alkylated. Proteins were precipitated with acetone and digested with either LysC and trypsin or using an S-trap microtube, then analyzed by LC-MS/MS. Data was searched with FragPipe. [doi:10.25345/C59C6SB5S] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: extracellular vesicles enrichment ; human plasma ; size exclusion chromatography ; SEC

Contact

Principal Investigators:
(in alphabetical order) 		Ernesto S. Nakayasu, Pacific Northwest National Laboratory, United States
Submitting User: alchemistmatt

Publications

Ivo Díaz Ludovico, Samantha M. Powell, Gina Many, Lisa Bramer, Soumyadeep Sarkar, Kelly Stratton, Tao Liu, Tujin Shi, Wei-Jun Qian, Kristin E Burnum-Johnson, John T. Melchior, Ernesto S. Nakayasu.
A fast and sensitive size-exclusion chromatography method for plasma extracellular vesicle proteomic analysis.
doi: 10.1101/2024.01.17.576135.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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