MassIVE MSV000100562

Partial Public

Trichoderma reesei QM6a SNI/BNI degradation experiment

Subscribe Comment Convert Spectra Add Reanalysis

Description

Goal :
Finding of bio-degradation products of several native SNIs and BNIs in Trichoderma reesei QM6a liquid minimal medium (12C and 13C) cultures.

Minimal medium
Amount for 500 mL medium | Actual | amount Substance
1.7500 g | 1.75115 g | (NH4)2SO4
2.5000 g | 2.51394 g | KH2PO4
0.6200 g | 0.63039 g | MgSO4 . 7 H2O
0.3125 g | 0.20447 g | NaCl
10 mL | 2 x 5 mL | 50X Trace element solution
ad 490 mL | 490 mL | autoclaved ELGA water
plus either 0.25g D-Glucose-13C or 0.25g D-Glucose-12C

Tested BNIs/SNIs (1.50mg each in D2O)
3,4-Dimethyl-1H-pyrazole phosphate (DMPP) [SOS 896]
Methyl p-coumerate (MHPA) [no SOS]
2-Amino-4-chloro-6-methylpyrimidine (ACMP) [no SOS]
Coixol (MBOA) [no SOS]
Karanjin
Sakuranetin
Syringic acid (SA) [SOS 899]
3,4-Dihydroxybenzaldehyde (DHBA) [SOS 900]
(R)-(+)-Limonene [SOS 277]

Trichoderma reesei QM6a spores on two MEX plates,
incubated with QM6a on 2024-08-22 (four days).
Transfer to BOKU IFA-Tulln.
Continued incubation at 28C, 90% relative humidity in constant darkness.

Inoculation and pre-cultivation:
Weighted glucose and filled up to respective volume with prepared minimal medium without carbon source
Sterile filtered prepare medium (at Lamina)
Inoculated medium with 15 uL spore solution per well (12C glucose) or 7 uL spore solution per well (U-13C6 glucose) respectively
Incubation at 28C, 70 % relative humidity in constant darkness in Memmert HPP260 Incubator

Treatment of cultures with SNI and BNI solutions and addition of more glucose

Sampling after 7 days

Measurements according to 10.1021/ac503290j, but with an Q Exactive HF instrument

[doi:10.25345/C5WS8J07J] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: biological nitrification inhibitor ; DatasetType:Metabolomics

Contact

Principal Investigators:
(in alphabetical order) 		christoph bueschl, BOKU university, Austria
Submitting User: christophb
Number of Files:
Total Size:
Spectra:
Subscribers:
 
Owner Reanalyses
Experimental Design
    Conditions:
    Biological Replicates:
    Technical Replicates:
 
Identification Results
    Proteins (Human, Remapped):
    Proteins (Reported):
    Peptides:
    Variant Peptides:
    PSMs:
 
Quantification Results
    Differential Proteins:
    Quantified Proteins:
 
Browse Dataset Files
 
FTP Download Link (click to copy):

Species

Instrument

Modifications

- Dataset Reanalyses

+ Dataset History

Click here to queue conversion of this dataset's submitted spectrum files to open formats (e.g. mzML). This process may take some time.

When complete, the converted files will be available in the "ccms_peak" subdirectory of the dataset's FTP space (accessible via the "FTP Download" link to the right).
Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.