MassIVE MSV000090907

Partial Public PXD038861

Mass spectrometry based identification of similarities and differences in protein content of cutaneous melanoma cell lines and exosomes

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Description

Intercellular transport of proteins mediated by extracellular vesicles (EVs) exosomes and ectosomes, is one of the factors facilitating carcinogenesis. Therefore, the research on protein cargo of melanoma-derived EVs may provide a better understanding of mechanisms related to melanoma progression and contribute to development of novel biomarkers. The data concerning the proteome of melanoma-derived EVs is very limited. We used the shotgun nanoLC-MS/MS approach to the profile protein content of primary (WM115, WM793) and metastatic (WM2664, WM1205Lu) cutaneous melanoma cell lines and exosomes. All cell lines secreted homogeneous populations of exosomes enriched in the common set of proteins. A total of 3514 and 1234 unique proteins were identified in melanoma cells and exosomes, respectively. Gene Ontology analysis showed that many of them were involved in cancer cell proliferation, migration, escape from apoptosis, epithelial mesenchymal transition and angiogenesis. The obtained results expand the knowledge about the role of selected proteins in the biology of exosomes, as well as their functional role in the development and progression of cutaneous melanoma. The results might also be used as a starting point for further studies exploring diagnostic and prognostic potential of exosomes. [doi:10.25345/C5F766C14] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: cancer ; extracellular vesicles ; exosomes ; melanoma ; proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Malgorzata Przybylo, Department of Glycoconjugate Biochemistry, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Poland
Submitting User: Urszula

Publications

Surman M, Jankowska U, Wilczak M, Przyby?o M.
Similarities and Differences in the Protein Composition of Cutaneous Melanoma Cells and Their Exosomes Identified by Mass Spectrometry.
Cancers (Basel). Epub 2023 Feb 8.

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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.