MassIVE MSV000084837

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LEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation

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Description

Quantitative crosslinking analysis to probe in vitro conformational dynamics governing ESCRT-mediated nuclear envelope formation. CHMP7 was crosslinked with D12-BS3 while CHMP7 in the presence of LEM2 winged helix domain was crosslinked with H12-BS3. Samples were mixed after crosslinking and analyzed according to standard procedures. The raw files included here correspond to four SEC fractions from a single experiment that contain crosslinked peptides. [doi:10.25345/C5339W] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: quantitative crosslinking mass spectrometry

Contact

Principal Investigators:
(in alphabetical order) 		Adam Frost, University of California San Francisco, USA
Submitting User: mtrnka

Publications

von Appen A, LaJoie D, Johnson IE, Trnka MJ, Pick SM, Burlingame AL, Ullman KS, Frost A.
LEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation.
Nature. 2020 Jun;582(7810):115-118. Epub 2020 Apr 29.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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