MassIVE MSV000090700

Complete Public PXD038115

Oncogenic pathways in uveal melanoma identified by phosphoproteomic analysis

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Description

The uveal melanoma cell lines MP41 and MP46 driven by oncogenic G11 (Q209L) and Gq (Q209L), respectively, and a control cell line OCM-1A driven by oncogenic BRAF (V600E) were treated for 24 hours with FR900359, a highly specific inhibitor of Gq/11. Protein extracts were collected and TMT labeled prior to LC-MS analysis. The enrichment of phosphopeptides from fractions of each sample was performed using IMAC prior to LC-MS. Proteomic and phospho-proteomic data analyses were performed to identify changes in post-translational modification in signaling pathways regulated by Gq/11 in these cells. [doi:10.25345/C5K06X54D] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Phosphoproteomics ; G-proteins ; Kinases ; Uveal melanoma ; FR900359

Contact

Principal Investigators:
(in alphabetical order) 		Kendall J. Blumer, Washington University in St. Louis, USA
Submitting User: jimmalone

Publications

Onken MD, Erdmann-Gilmore P, Zhang Q, Thapa K, King E, Kaltenbronn KM, Noda SE, Makepeace CM, Goldfarb D, Babur Ö, Townsend RR, Blumer KJ.
Protein Kinase Signaling Networks Driven by Oncogenic Gq/11 in Uveal Melanoma Identified by Phosphoproteomic and Bioinformatic Analyses.
Mol Cell Proteomics. 2023 Nov;22(11):100649. Epub 2023 Sep 19.

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