MassIVE MSV000099031

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Engineering unnatural cells with a 21st amino acid as a living epigenetic sensor

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Description

Living animals rely extensively on posttranslational modifications (PTMs) to regulate protein activity, stability, subcellular localization, and protein protein interactions. These modifications are tightly controlled by the balance of writer and eraser enzymes, which add or remove PTMs on proteins. Current strategies to measure writer and eraser activities in living animals largely depend on invasive methods such as single cell sequencing, quantitative mass spectrometry, or activity based probes, which often lack cell or tissue specificity. In this study, we report the development of autonomous cells, both prokaryotic and eukaryotic, with the ability to biosynthesize and genetically encode acetyllysine using genetic code expansion technology. These engineered living sensors with a site specific acetyllysine modification can be transplanted into living animals, enabling real time monitoring of PTM dynamics in living cells and their visualization within animal models. We further demonstrate the utility of these cells in tracking deacetylase activity and assessing the effects of deacetylase inhibitors on PTM dynamics in living animals. [doi:10.25345/C5J960P4R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: ncAA AcK GCE biosynthesis sensor Sirtuins ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Han Xiao, Rice University, United states
Submitting User: yuhu
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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