MassIVE MSV000095018

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Proteomic and phosphoproteomic dataset of human FFPE lung adenocarcinomas with mutations

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Description

Mutations in key oncogenes have been identified as important genetic alterations in lung adenocarcinoma (LUAC), including genes encoding epidermal growth factor receptor (EGFR), Kirsten rat sarcoma viral oncogene homolog (KRAS), and anaplastic lymphoma kinase (ALK). These genetic mutations fundamentally determine the therapeutic options of the patients. Our aim was the characterization of the altered biological pathways and phosphorylation events in LUAC tumors harboring EGFR, KRAS, or ALK oncogenic mutation and triple wild-type (WT) LUAC tumors by MS-based quantitative proteomics and phosphoproteomics analyses of 84 formalin-fixed paraffin-embedded (FFPE) tissue sections. EGFR-mutated samples showed distinct proteomic profiles, while principal component analysis based on the phosphoproteomic experiments revealed considerable differences between the samples with different mutations. Additionally, 31 proteins and 60 phosphosites were identified with potentially mutation-specific values serving as potential therapeutic targets. Our results could provide significant insights for developing therapeutic strategies for LUAC. [doi:10.25345/C5W37M669] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Phosphoproteomics ; Proteomics ; MS ; Lung adenocarcinoma ; FFPE tissue ; Cancer research ; Kinase-substrate enrichment analysis

Contact

Principal Investigators:
(in alphabetical order) 		Lilla Turiak, MTA-TTK Lendulet (Momentum) Glycan Biomarker Research Group, HUN-REN Research Centre for Natural Sciences, Hungary
Submitting User: BugyiFanni
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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