MassIVE MSV000096100

Complete Public PXD056822

Dessauer_Adcy_TripleTOF6600_P124

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Description

This dataset consists of 32 raw BioID MS files and 8 raw FLAG-AP samples and associated peak lists and results files, acquired on a ABSciex TripleTOF6600 operated in Data Dependent Acquisition mode. Samples were generated by Taeyeop Park. Streptavidin affinity purification was performed by Zhen-Yuan Lin, anti-FLAG affinity purification performed by Cassandra Wong. Mass spectrometry acquisition was performed by Zhen-Yuan Lin and Cassandra Wong. Analysis was performed by Taeyeop Park, Cassandra Wong, Karen Colwill, and Carmen Dessauer. The files are associated with a manuscript submitted for publication by Taeyeop Park et al. The main goal of this dataset was to identify isoform-specific adenylyl cyclase-containing complexes in neonatal cardiomyocytes. Carmen W. Dessauer (Carmen.W.Dessauer@uth.tmc.edu) is the corresponding authors of the manuscript; Karen Colwill should be contacted for questions on this dataset (colwill@lunenfeld.ca) This submission is associated with 6 Supplementary Files (in addition to this README file) Table 1 BioID describes the composition of the BioID dataset Table 1 FLAG-AP describes the composition of the FLAG-AP dataset Table 2 BioID lists all the peptide identification evidence of the BioID dataset Table 2 FLAG-AP lists all the peptide identification evidence of the FLAG-AP dataset Table 3 BioID lists the SAINTexpress interactions for BioID dataset Table 3 FLAG-AP lists the SAINTexpress interactions for FLAG-AP dataset [doi:10.25345/C5M32NN5P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Affinity purification ; BioID ; protein-protein interaction ; Adenylyl cyclase ; cardiomyocytes ; Adcy ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Karen Colwill, LTRI, Canada
Submitting User: LTRI_proteomics
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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.