MassIVE MSV000091181

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Raw mas spectra of bacterial proteins

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Description

Bovine mastitis is the most common disease that affects dairy cattle worldwide and generates millions of losses for cattle breeders. One of the most common pathogens identified in infected milk samples is Staphylococcus aureus. Presently, there is no fast, bacterial species-recognizing test in the diagnostic market. The aim of this study was bioinformatic detection and characteristic of fibronectin binding protein A (FnBPA) of Staphylococcus aureus (SA) and investigation of this protein in milk samples obtained from cows with diagnosed mastitis. Bioinformatic detection of potential biomarker for bovine SA obtained analyses of >90.000.000 amino acid sequences. Analyses on FnBPA included, among others: detection of signal peptides, non-classical proteins, antigenicity, and epitopes prediction. To confirm a presence of the fnbA gene in four SA isolates amplification with specific primers was performed. Detection of FnBPA was carried out by immunoblotting. Proteins mixture was separated by SDS-PAGE, and immunoreactivity and selectivity were performed by monoclonal anti-FnBPA antibodies and SA-negative serum. Bioinformatic analyses showed that FnBPA is a surface, conservative, immunoreactive, and species-specific protein with antigenic potential. Molecular methods confirmed its presence in 100% of isolates, and immunoblotting proved its immunoreactivity and specificity. Thus, it can be considered as potential biomarker in immunodiagnostic of mastitis. [doi:10.25345/C5Q815280] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: protein ; mas spectra ; bacteria ; mastitis

Contact

Principal Investigators:
(in alphabetical order) 		Anna Dobrut, Jagiellonian University in Krakow, Poland
Dorota Pietras-Ozga, University of Life Science in Lublin, Poland
Katarzyna Michalak, University of Life Science in Lublin, Poland
Submitting User: Dorota_Ozga1983
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.