MassIVE MSV000087853

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Pseudomonas savastanoi pv phaseolicola R5 treated with salicylic acid

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Description

Tags 126, 127N, and 128C were assigned to control replicates, 127C, 128N, and 130C were assigned to 0.5 mM SA-treated replicates, and 129C, 130N, and 131 were assigned to 1.0 mM SA-treated replicates. Labeled samples were mixed together and fractionated by high-pH reverse phase HPLC. Twelve concatenated fractions were analyzed. Peptides from each fraction were separated using a gradient of 3 to 27% of 90% acetonitrile\0.1% formic acid over 180 minutes and were electrosprayed at 2.6 kV into an Orbitrap Fusion mass spectrometer operating in data-dependent mode with positive polarity. Quadrupole isolation was enabled, and survey scans were recorded in the Orbitrap at 120,000 resolution over a mass range of 400-1500 m/z. The automatic gain control (AGC) target was set to 1,600,000 and the maximum injection time was set to 50 milliseconds (msec). The most abundant precursor ions (intensity threshold 5,000) were fragmented by collision-induced dissociation (35% energy) and fragment ions were detected in the linear ion trap (AGC 32,500, 50 msec maximum injection). Analyzed precursors were dynamically excluded for 150 seconds. Multiple MS2 fragment ions were captured using isolation waveforms with multiple frequency notches and fragmented by high energy collision-induced dissociation (65% normalized collision energy). MS3 spectra were acquired in the Orbitrap (AGC 250,000; maximum injection time 200 msec, 50,000 resolution scanning from 100-1000 m/z). [doi:10.25345/C5SZ6K] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: salicylic acid

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Principal Investigators:
(in alphabetical order) 		Bret Cooper, USDA-ARS, United States
Submitting User: cooperb
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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