MassIVE MSV000085243

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PomGnt1 deficiency affects N-cadherin mediated cell-cell adhesion

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Description

N-glycoproteomic and O-man glycoproteomic analysis of N-cadherin derived from wild-type(WT) and POMGnT1-deficient HEK293T cells. Proteolytic digestion was performed with trypsin or proteinase K. Glycopeptide enrichment was achieved using cotton-HILIC SPE. Glycopeptides were fragmented by HCD.step and HCD.low. For O-man glycoproteomics N-glycan release by PNGase F was included. [doi:10.25345/C5TB0D] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: cell-cell adhesion, N-glycosylation, O-Mannosylation

Contact

Principal Investigators:
(in alphabetical order) 		Marcus Hoffmann, Max Planck Institute for Dynamics of Complex Technical Systems, Germany
Submitting User: mhoffmann
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