MassIVE MSV000097130

Partial Public

1. Mitochondrial Protein Identification; 2. Redox Proteomics Identification; 3. Quantitative Cellular Proteomics

Description

1. Quantitative Proteomic Analysis of Isolated Mitochondria. Each group has three replicates: Glc group: Glucose starvation group. GlcFH group: FH activity inhibition combined with glucose starvation group. 2. Quantitative proteomics analysis was performed to assess intracellular protein disulfide bond levels. The experiment included the following groups, each with three replicates:UOK group: UOK262 cells under glucose starvation; UOKFH group: UOK262 cells overexpressing FH under glucose starvation. V786O group: 786-O cells under glucose starvation; XCT786O group: 786-O cells overexpressing XCT under glucose starvation. 3. Quantitative Proteomics Analysis of Cellular Protein Expression Differences, Each group has three replicates: Glcn group: glucose starvation group. Glcp group: glucose sufficiency group [doi:10.25345/C5H41K02X] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Mitochondrial, Redox, Quantitative, Cellular, Proteomics, Disulfide Stress ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order)
Guowang Xu, DICP, China
Submitting User: luxin722
Number of Files:
Total Size:
Spectra:
Subscribers:
 
Owner Reanalyses
Experimental Design
    Conditions:
    Biological Replicates:
    Technical Replicates:
 
Identification Results
    Proteins (Human, Remapped):
    Proteins (Reported):
    Peptides:
    Variant Peptides:
    PSMs:
 
Quantification Results
    Differential Proteins:
    Quantified Proteins:
 
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.