MassIVE MSV000081976

Imported Reanalysis Dataset Public PXD006063

Chinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 4

Description

Chinese Hamster Ovary (CHO) cells are frequently used to produce recombinant HIV envelope protein gp120. In this study, we characterized the CHO proteins that become co-purified with gp120 when lectin affinity chromatography is used. Many of co-purified CHO proteins identified were extracellular matrix components. We verified a method for separating gp120 from these CHO proteins with anion exchange chromatography, and assessed the biochemical activity of gp120 preparations with and without co-purified CHO proteins. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: HIV ; gp120 ; fibronectin ; extracellular matrix ; CHO ; Chinese Hamster

Contact

Principal Investigators:
(in alphabetical order)
Shiu-Lok Hu, Unversity of Washington, N/A
Submitting User: ccms

Publications

Plotnik D, Guo W, Cleveland B, von Haller P, Eng JK, Guttman M, Lee KK, Arthos J, Hu SL.
Extracellular Matrix Proteins Mediate HIV-1 gp120 Interactions with ?4?7.
J. Virol. Epub 2017 Oct 13.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.