MassIVE MSV000092581

Complete Public PXD044317

Comparative analysis of differential cellular genome and proteome regulation by HIV-1 and HIV-2 pseudovirions in the early phase of infection

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Description

Beside the similar structure and genomic organization of human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2), striking difference exist between the in terms of replication dynamics and clinical manifestation of infection. Although pathomechanism of HIV-1 infection well characterized, relatively few data are available regarding HIV-2 viral replication, and its interaction with host cell protein during the early phase of infection. We utilized proteo-transcriptomic analyses to determine differential genome expression and proteomic changes induced by transduction with HIV-1 / 2 pseudovirions during eight, 12, and 26 hour time-points in HEK-293T cells. We show that alteration in the cellular milieu was indeed different between the two pseudovirions. The significantly higher number of genes altered by HIV-2 in the first two time-points, suggest a more diverse, yet subtle effect on the host cell, preparing the infected cell for integration and latency. On the other hand, GO analysis showed that, while HIV-1 induced cellular oxidative stress and had a greater effect on the cellular metabolism, HIV-2 mostly affected genes involved in cell adhesion, extracellular matrix organization or keratinocyte differentiation. Proteomics analysis revealed that HIV-1 upregulated, while HIV-2 downregulated proteins involved viral protein expression and replication. Our study provides an insight into the understudied replication cycle of HIV-2, and enrich our knowledge about the use of HIV-based lentiviral vectors in general. [doi:10.25345/C5CN6Z90C] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: HIV1, HIV2, proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Gergo Kallo, University of Debrecen, Hungary
Submitting User: kallogergo

Publications

Linkner TR, Ambrus V, Kunkli B, Szojka ZI, Kalló G, Cs?sz É, Kumar A, Emri M, T?zsér J, Mahdi M.
Comparative Analysis of Differential Cellular Transcriptome and Proteome Regulation by HIV-1 and HIV-2 Pseudovirions in the Early Phase of Infection.
Int J Mol Sci. Epub 2023 Dec 27.

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