MassIVE MSV000100825

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Proteomic analysis of X19728 exposure in triple-negative breast cancer (TNBC) cells

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Description

To define the downstream protein-level consequences of X19728 exposure in triple-negative breast cancer (TNBC) cells, wild-type and AHR-knockout MDA-MB-468 cells were treated with vehicle control (DMSO) or 3 uM X19728 (2-phenyl-imidazo[1,2-a]pyridine) for 36 hours. Following treatment, whole-cell lysates were collected. Proteins were extracted, reduced, alkylated, and digested with trypsin prior to analysis using an Evosep One liquid chromatography system coupled to a timsTOF HT mass spectrometer (Bruker). [doi:10.25345/C5251G001] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Proteomics ; Triple-Negative Breast Cancer Cells ; MDA-MB-468 ; ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Siva Kolluri, Oregon State University, United states
Submitting User: ebrahiar
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Identification Results
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Quantification Results
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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