MassIVE MSV000093152

Partial Public PXD046240

Shotgun proteomics of thyroid carcinoma exosomes: insight into the role of exosomal proteins in carcinogenesis and thyroid homeostasis

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Description

The transport of molecules via exosomes is one of the factors involved in cancer development, and transferred molecules can serve as specific tumor biomarkers. The aim of the study was the LC MS/MS proteomic analysis of exosomes released by FTC and 8305C thyroid cancer cell lines, and reference Nthy-ori 3-1 normal thyroid follicular cells. A total of 1769 unique proteins were identified in the exosome samples. For exosomes derived from Nthy ori 3 1 cell the highest number of 1504 proteins was identified, while exosomes from thyroid carcinomas FTC and 8305C cell lines had 730 and 1304 identified proteins, respectively. For the identified proteins, gene ontology analysis was performed in terms of their cellular location, molecular function and involvement in biological processes. For proteins that only appeared in tumor derived FTC- and 8305C-derived exosomes, enriched categories were related to cancer progression and included cell adhesion and positive regulation of cell migration. Also, proteins related to protein N linked glycosylation, drug resistance, and cell response to NK and T cell cytotoxicity were among those unique proteins. Finally label free quantification (LFQ) was performed for identified differentially expressed proteins between all possible group parings. For exosomes from thyroid cancer cells, the most differentiating proteins included collagen alpha 2(I) chain, tenascin, matrix metalloproteinase 1, Interstitial collagenase and C type lectin domain family 11 member A. The obtained results broadened the knowledge about the role of exosomal proteins in thyroid cancer and indicated potential biomarkers for further evaluation in clinical conditions. [doi:10.25345/C5ZG6GJ1R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: biomarkers ; exosomes ; thyroid cancer

Contact

Principal Investigators:
(in alphabetical order) 		Malgorzata Przybylo, Department of Glycoconjugate Biochemistry, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Poland
Submitting User: Urszula

Publications

Surman M, Wilczak M, Jankowska U, Skupie?-Rabian B, Przyby?o M.
Shotgun proteomics of thyroid carcinoma exosomes - Insight into the role of exosomal proteins in carcinogenesis and thyroid homeostasis.
Biochim Biophys Acta Gen Subj. 2024 Sep;1868(9):130672. Epub 2024 Jul 16.

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