MassIVE MSV000097470

Partial Public PXD067954

CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation

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Description

HEK293T cells were co-transfected with StrepII-FLAG-tagged DCAF12 and HA-tagged CUL4A, grown for 48 h, harvested, and lysed as described above. In the first step, Strep-TactinXT Superflow resin (IBA Lifesciences, Gottingen, Germany) was used to purify proteins associated with StrepII-FLAG-tagged subsrtrate receptors. In the second step, eluates from the previous step were used for immunoprecipitation of HA-CUL1/4A-associated proteins. Eluates from both steps were analyzed by LC-MS/MS at the Proteomics facility of Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University (BIOCEV) in Vestec, Czech Republic. Further details are provided in Supplementary methods. [doi:10.25345/C5Q23RC2V] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: ubiquitin, proteasome ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Lukas Cermak, Institute of Molecular Genetics of the ASCR, v. v. i., Czech republic
Submitting User: Lukascermak

Publications

Lidak T, Baloghova N, Korinek V, Sedlacek R, Balounova J, Kasparek P, Cermak L.
CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation.
Int J Mol Sci. Epub 2021 May 20.

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