MassIVE MSV000087458

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TMT MS analysis of the total proteome of NUMB exon 9 deleted MDA_MB_468 cells

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Description

This experiment is to study the molecular mechanism of the Numb exon 9 containing isoform by quantitative measurement of the change in total proteome expression in response to NUMB exon 9 deletion. Total protein lysate from two MDA-MB-468 control cell pools (n = 4) and three NUMB exon 9 deleted cell pools (n = 6) from independent targeting events were collected. Each cell pool has two replicates which were separately seeded. [doi:10.25345/C5SZ56] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: quantitative proteomics ; isoform ; NUMB

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Principal Investigators:
(in alphabetical order) 		Jane McGlade, Hospital for Sick Children, Canada
Submitting User: alicezhang
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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