MassIVE MSV000091565

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Proteome-Wide Identification of RNA-Dependent Proteins: An Emerging Role for RNAs in Plasmodium falciparum Protein Complexes

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Description

Ribonucleoprotein complexes are composed of RNA, RNA-dependent proteins (RDPs) and RNA-binding proteins (RBPs), and play fundamental roles in RNA regulation. However, in the human malaria parasite, Plasmodium falciparum, identification and characterization of these proteins are particularly limited. In this study, we use an unbiased proteome-wide approach, called R-DeeP, a method based on sucrose density gradient ultracentrifugation, to identify RDPs. Quantitative analysis by mass spectrometry identified 785 RDPs, including 463 proteins newly associated with RNA. Results were further validated using a combination of computational, cellular, and genetic assays. Overall, this method provides the first snapshot of the Plasmodium protein-protein network in the presence and absence of RNA. R-DeeP also helps to reconstruct Plasmodium multiprotein complexes based on co-segregation and deciphers their RNA dependence. [doi:10.25345/C5697075P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: malaria ; Plasmodium ; R-DeeP ; RNA-binding proteins

Contact

Principal Investigators:
(in alphabetical order) 		Karine Le Roch, University of California, Riverside, USA
Submitting User: THOLLIN
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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