MassIVE MSV000091949

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Syncytin1 sequesters viral LF2 to enhance the Epstein-Barr virus lytic phase

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Description

Previously, we identified Syncytin1 to be expressed in human B cells and enhance EBV ability to undergo lytic replication. To gain mechanistic insight, we performed liquid chromatography tandem mass spectrometry (LC-MS/MS) on tryptic peptides from immunoprecipitated FLAG-tagged Syncytin-1 in latently infected EBV+ Burkitt lymphoma cells. This revealed a number of cellular proteins as potential Syncytin-1 interacting partners during latency and/or during the lytic phase as well as two EBV lytic phase proteins, the viral protein kinase and LF2, a negative regulator of EBV lytic gene transcription. We now find that Syncytin1 reduces the association between LF2 and the EBV replication and transcription activator to promote EBV lytic gene expression. [doi:10.25345/C5KH0F85B] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: EBV

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Principal Investigators:
(in alphabetical order) 		Michael T. McIntosh, Ph.D., University of Florida, USA
Submitting User: jhaley55
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