MassIVE MSV000086870

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GNPS Colostethus imbricolus - SBC18 column

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Description

All analysis were performed in reversed-phase HPLC Zorbax SB-C18 column (150 mm x 2.0 mm x 3.0 um, Agilent technologies, Santa Clara, USA) for optimizing the separation conditions. The mobile phases were A (0.1% v/v formic acid in Milli-Q water) and B (0.1% v/v formic acid in ACN) at a flow rate of 0.3 mL/min and 45ºC. Gradient elution started to 5%B for 2 min, then increased to 20%B at 4 min, then to 30%B at 15 min, 34%B at 25 min, 45%B at 30, and finally to 100%B at 45 min and maintained for 5 min and thereafter returned back to starting conditions in 2 min and finally 13 min of re-equilibration time was applied. Total run time was 65 min. MS spectra were acquired in positive ion mode in the range of 20–2000 m/z and a scan rate of 6 scan/min. 10 uL of each frog extract was injected by duplicate using an the same HPLC-Q-TOF system (Agilent Technologies, Santa Clara, CA, USA) employed for the targeted analysis. Mass range for the full scan was performed between 20 to 2000 m/z, nebulizer gas flow rate of 8 L/min, pressure of 10 psi, and fragmentor of 175 V. The mass correction was performed on the same way that for the targeted analysis. For MS/MS analysis, auto MS/MS acquisition mode was employed for the analysis of one of the samples employing a mass range between 100 to 3000 m/z, a scan rate of 6.0 scans/s, isolation width of 1.3 amu, [doi:10.25345/C5SZ22] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Poison frogs ; Dendrobatoidea ; TTX ; Alkaloids

Contact

Principal Investigators:
(in alphabetical order) 		Mabel Gonzalez, Uniandes, Colombia
Submitting User: mabel_gonzalez
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GNPS content goes here (MSV000086870 [task=c2782defa98d4b37a43565b1af7a269b])
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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