MassIVE MSV000087150

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Proteomics on the ovaries of failed and healthy honey bee queens

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Description

We sampled honey bee queens from beekeepers throughout British Columbia which were rated as either 'failed' or 'healthy' by beekeepers. We performed shot-gun proteomics on the ovaries. Proteins were reduced, alkylated, and digested with Lys-C for 4 h in urea digestion buffer. The sample was then diluted 4x with ammonium bicarbonated and digested overnight with trypsin. Peptides were analyzed by an EASY-nLC 1000 liquid chromatography system coupled to a Bruker Impact II mass spectrometer. Data were searched with MaxQuant and the zipped txt folder output is provided. [doi:10.25345/C55V46] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: honey bee ; apis mellifera ; ovaries ; queen

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Principal Investigators:
(in alphabetical order) 		Leonard Foster, University of British Columbia, Canada
Submitting User: abbichapman
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