MassIVE MSV000084266

Partial Public PXD015261

TMT10 controlled mixtures - SILAC HeLa UPS1, acquired using MS2-only strategy

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Description

The controlled mixtures were prepared to evaluate the ability of our proposed approach when dealing with the complicated design, e.g. multiple TMT mixtures with technical replicates. 500, 333, 250, and 62.5 fmol UPS1 peptides were spiked-into 50 g SILAC HeLa peptides in duplicate. It produced a dilution series corresponding to 1, 0.667, 0.5, and 0.125 of the highest UPS1 peptide amount (500 fmol). In addition, a reference sample was generated by pooling all four diluted UPS1 peptide samples (286.5 fmol) and combined with 50 g of SILAC HeLa in duplicate. These 10 replicates were labeled with TMT10-plex reagents and mixed together to pass LC-MS/MS analysis. The procedure was repeated to generate a total of five such controlled mixtures. To assess technical variability, three technical replicates were prepared for each mixture. Totally there are 15 MS runs from 5 TMT mixtures. LC-MS/MS was performed using an EASY-nLC 1200 ultrahigh pressure liquid chromatography (UHPLC) connected to an Orbitrap Fusion Lumos Tribrid and equipped with an EASY-spray source (Thermo Fisher Scientific, San Jose, CA). The same samples for MSV000084264 (or PXD0015258) are used. The data were acquired using MS2-only strategies. [doi:10.25345/C53M2D] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: TMT10plex ; Controlled Mixture ; Lumos ; Proteomics ; MassIVE.quant reviewed - Platinum

Contact

Principal Investigators:
(in alphabetical order) 		Olga Vitek, Khoury College of Computer Sciences, Northeastern University, United States
Tom Dunkley, Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Basel, Hoffmann-La Roche Ltd, Switzerland
Submitting User: mnchoi
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