MassIVE MSV000091583

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GlycoNote with iterative decoy searching and open-search component analysis for high-throughput and reliable glycan spectral interpretation--Supplementary-Data

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Description

Mass spectrometry-based glycome analysis is a viable strategy for the compositional and functional exploration of glycosylation. However, the lack of generic tools for high-throughput and reliable glycan spectral interpretation largely hampers the broad usability of glycomic research. Here, we develop a generic and reliable glycomic tool, GlycoNote, for comprehensive and precise glycome analysis. GlycoNote supports interpretation of any type of tandem-mass spectrometry glycomic data, performs a novel target-decoy method with iterative decoy searching for highly reliable result output, and embeds an open-search component analysis mode for heterogeneity analysis of monosaccharides and modifications. We test GlycoNote on several different large-scale glycomic datasets, including human milk oligosaccharides, N- and O-glycome from human cell lines, plant polysaccharides, and atypical glycans from Caenorhabditis elegans, demonstrating its high capacity for glycome analysis. An application of GlycoNote to the analysis of labeled and derived glycans further demonstrates its broad usability in glycomics studies. By enabling generic characterization of various glycan types and elucidation of component heterogeneity in glycomic samples, the freely available GlycoNote is a promising tool for facilitating glycomics in glycobiology research. [doi:10.25345/C5VT1H061] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: GlycoNote ; glycan spectral

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Principal Investigators:
(in alphabetical order) 		Weiqian Cao, Institutes of Biomedical Sciences,Fudan University, China
Submitting User: wqcao
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