Description
Saliva was collected from n=3 subjects, before and after treatment with octreotide. 100 micrograms of saliva protein was reduced and alkylated, and digested with trypsin using an S-trap. Peptides (~45 ugs) were separated by microflow LC using a 1x100 mm Waters ACQUITY Premier CSH column at 100 uL/min using a gradient of 3-258 MeCN over 60 min. 3% DMSO was delivered post-column. The LC was interfaced to a Thermo Exploris 480 using a heated ESI source. MS/MS analysis used a staggered, overlapping DIA method with 60 K MS1 and 15K MS2 spanning 400-1000 m/z with 77 x 16 m/z windows and a 36 window cycle. Data was processed using Spectronaut Pulsar 15.
[doi:10.25345/C5639K83W]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: microflow ; data-independent acquisition
Contact
Principal Investigators:
(in alphabetical order)
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Walter T. Lee, Duke University, USA
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mwfoster
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Publications
Jonathan Cohen, William Reed, Matthew W. Foster, Russel R. Kahmke, Daniel J. Rocke, Liana Puscas, Trinitia Y. Cannon, Walter T. Lee.
Octreotide may improve pharyngocutaneous fistula healing through downregulation of cystatins: A pilot stud.
Laryngoscope Investigative Otolaryngology. 2022;1?7. doi:10.1002/lio2.962.
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Conditions:
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Technical Replicates:
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| Identification Results |
Proteins (Human, Remapped):
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Proteins (Reported):
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Peptides:
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Differential Proteins:
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Quantified Proteins:
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Number of distinct conditions across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct condition labels are counted across all files submitted in the "Metadata" category
having a "Condition" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct replicate labels are counted across all files submitted in the "Metadata" category
having a "BioReplicate" or "Replicate" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses)
associated with this dataset.
The technical replicate count is defined as the maximum number of times any one distinct
combination of condition and biological replicate was analyzed across all files submitted in the
"Metadata" category. In the case of fractionated experiments, only the first fraction is
considered.
"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original
submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported
across all analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.