MassIVE MSV000097257

Partial Public PXD061555

Cell therapy mesenchymal stem cell secretome for cardioprotection

Subscribe Comment Reanalyze Spectra Add Reanalysis

Description

Mesenchymal stem cells (MSCs) offer promising therapeutic effects for cardiac repair, primarily through their secretome, a complex array of bioactive factors with broad, multi-target effects on various cell types and pathological processes. However, current delivery and retention methods lack the ability to provide sustained, stable therapeutic benefits in ischaemic heart disease. This study addresses this limitation by introducing an innovative approach for the prolonged and clinically feasible delivery of MSC-derived secretome for cardioprotection. To evaluate the cardioprotective effects of MSC secretome in a human context, a human-iPSC-derived cardiac organoid model of simulated ischaemia-reperfusion injury was employed. Proteomic analysis of the Cymerus secretome, collected at both pre- and post-implantation, was conducted to investigate the mechanisms underlying its cardiac benefits. Proteomic analysis revealed the secretome of Cymerus comprised 3,851 proteins, with functional enrichment in pathways related to tissue homeostasis, apoptosis regulation, wound healing, and antioxidant defence . Furthermore, the post-implantation MSC secretome demonstrated an upregulation of proteins related to extracellular matrix organization, immune modulation, and tissue remodelling, indicating a temporal and adaptive response of the MSCs to ischaemic conditions. [doi:10.25345/C53B5WM4J] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: heart ; secretome ; therapeutic ; stem cells ; Ischaemia-reperfusion injury ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		David Greening, Baker Heart & Diabetes Institute, Australia
Submitting User: dwgree
Number of Files:
Total Size:
Spectra:
Subscribers:
 
Owner Reanalyses
Experimental Design
    Conditions:
    Biological Replicates:
    Technical Replicates:
 
Identification Results
    Proteins (Human, Remapped):
    Proteins (Reported):
    Peptides:
    Variant Peptides:
    PSMs:
 
Quantification Results
    Differential Proteins:
    Quantified Proteins:
 
Browse Dataset Files
 
FTP Download Link (click to copy):

Species

Instrument

Modifications

- Dataset Reanalyses

+ Dataset History

Click here to queue conversion of this dataset's submitted spectrum files to open formats (e.g. mzML). This process may take some time.

When complete, the converted files will be available in the "ccms_peak" subdirectory of the dataset's FTP space (accessible via the "FTP Download" link to the right).
Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.