MassIVE MSV000084058

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Global proteomic analyses of STING-positive and -negative macrophages reveal STING and non-STING differentially regulated cellular and molecular pathways

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Description

The STING signaling pathway plays important roles in many cancers, viral and bacterial infections, and autoimmune diseases. Understanding how STING mediates expression of many proteins in the host and bacterial cells can provide important clues about the biochemical processes, opening the door for the development of new therapies. Using STING KO cells, we were able to identify many differentially expressed proteins between the wild type and STING KO RAW macrophage. We identified proteins that were exclusively detected only in the wild type or only in the STING KO, as well as those that were comply identified but showed different levels of expressions between the two cell lines. This included several proteins that are known as potent biomarkers of various cancers and autoimmune diseases. Our data broaden the knowledge of proteins that are related to STING signaling, and may have identified novel proteins that have therapeutic value. This will require rigorous validation in the future studies. Many protein biomarkers can be regulated by post-translational modifications and protein-protein interactions. While beyond the scope of the current study, we note that methods are available at the Purdue Proteomics Facility for mapping post-translational modifications and protein-protein interactions. We envision this to be an exciting line of enquiry for us in the future. [doi:10.25345/C56Q10] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Label free MS1 quantitation ; proteomics ; Mass Spectrometry ; RAW macrophage ; STING knock out

Contact

Principal Investigators:
(in alphabetical order) 		Prof. Herman Sintim, Purdue University, USA
Submitting User: uma_aryal
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