MassIVE MSV000098309

Partial Public PXD065413

Bacteria covalently incorporate polyfluoroalkyl carboxylates into membrane lipids

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Description

Per- and polyfluoroalkyl substances (PFASs) are global contaminants of concern to human and environmental health 1. Little is known about biological attenuation processes and microbiota PFAS interactions. Here we show that the soil bacterium Pseudomonas sp. strain 273 covalently incorporates polyfluoroalkyl carboxylates (FTCAs) including 8:3 FTCA into phosphatidylethanolamine (PE) and phosphatidylglycerol (PG), two prominent components of lipid bilayers. Semi-quantitative lipidomic analysis estimated that 7.5% of the bacteriums phospholipid pool contained 8:3 FTCA, resulting in a PFAS membrane incorporation factor (MIF) of 423. Synthesis of PE and PG species carrying a polyfluorocarbon acyl tail was observed in five other axenic bacterial cultures tested, albeit with lower MIFs. Our results suggest that the formation of fluoromembranes is a prevalent bacterial process potentially impacting the fate, longevity, bioaccumulation, and biomagnification of certain PFASs. [doi:10.25345/C5SB3XB59] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: PFASs ; polyfluoroalkyl carboxylates ; fluoromembranes ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Paul Abraham, ORNL, United States
Submitting User: dlcarper
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